spoIIIE [2018-02-11 19:22:24]

ATP-dependent dsDNA translocase, resolution of chromosomal dimers after DNA replication, transports the forespore chromosome across the sporulation septum

locus: BSU16800

pI: 6.76

mw: 86.96 kDa

protein length: 787 aa

gene length: 2361 bp

function: chromosome partition during sporulation

product: ATP-dependent dsDNA translocase required for chromosome partition

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.3|Sporulation/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,752,272 → 1,754,641

The protein

Catalyzed reaction/ biological activity

• transports the forespore chromosome across the sporulation septum [Pubmed|20516200]
• translocates septum-entrapped DNA only when septum closure precedes complete segregation of chromosomes [Pubmed|19818024]
• the two DNA translocases [protein|DF77B46666595E86081C5345C91E08F86DF9FAEE|SftA] and [protein|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|SpoIIIE] synergistically affect chromosome dimer resolution presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively [Pubmed|21239579]
• binds DNA sequences named SRS for SpoIIIE recognition sequence, GAGAAGGG [Pubmed|18391964]

Paralogous protein(s)

• [protein|DF77B46666595E86081C5345C91E08F86DF9FAEE|SftA]

[SW|Domains]

• N-terminal transmembrane domain (4 helices) [Pubmed|24297254]
• 134 aa linker [Pubmed|24297254]
• 512 aa motor domain at the C-terminus [Pubmed|24297254]

Structure

• [PDB|2IUT] (FtsK from ''Pseudomonas aeruginosa'', partial, 48% identity, complex with [protein|DF77B46666595E86081C5345C91E08F86DF9FAEE|SftA]) [Pubmed|16916635]

[SW|Localization]

• transmembrane domain mediates dynamic localization to active division sites [Pubmed|20516200]
• complexes are recruited to nascent sites of septation, and are subsequently escorted by the constriction machinery to the center of [SW|sporulation] and division septa [Pubmed|23667326]

Expression and Regulation

Operons

• genes: [gene|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|spoIIIE]-[gene|F9E0C645BCCD9A299E951D0574321C1FACA8DE4C|ymfC]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2507870], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• BKE16800 (Δ[gene|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|spoIIIE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACACTCATCACCTTACTGC, downstream forward: _UP4_TAATGAAGGGAGTTCCGCTT
• BKK16800 (Δ[gene|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|spoIIIE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACACTCATCACCTTACTGC, downstream forward: _UP4_TAATGAAGGGAGTTCCGCTT

References

Reviews

• 22047950,22934648,23400100,25460803

Original publications

• 18160039,18593879,3129532,2507870,18593879,23559069,9150232,2492502,11062134,9155041,14680637,7797072,7567988,2507870,8160014,9515703,19818024,19788545,20298190,20516200,21239579,18391964,23667326,11134515,24297254,24769697,25950186,26452092,16916635,26849443,27886417,27891124